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phospho akt  (R&D Systems)


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    Structured Review

    R&D Systems phospho akt
    Cyclic mechanical stretch loading induces <t>AKT</t> phosphorylation (p-AKT at <t>Ser473)</t> in adipocytes. Cyclic stretching at 5% strain and 1 Hz frequency was applied to differentiated 3T3-L1 adipocytes for 2 h. ( A ) Immunoblots of AKT and p-AKT with GAPDH loading control. ( B ) After normalization with GAPDH, relative immunoblot band intensities of p-AKT/AKT were obtained. Mean ± SEM; *: p < 0.05 (n = 3) by Student t -test.
    Phospho Akt, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 84 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho akt/product/R&D Systems
    Average 93 stars, based on 84 article reviews
    phospho akt - by Bioz Stars, 2026-02
    93/100 stars

    Images

    1) Product Images from "Mechanical Stretch Control of Adipocyte AKT Signaling and the Role of FAK and ROCK Mechanosensors"

    Article Title: Mechanical Stretch Control of Adipocyte AKT Signaling and the Role of FAK and ROCK Mechanosensors

    Journal: Bioengineering

    doi: 10.3390/bioengineering11121279

    Cyclic mechanical stretch loading induces AKT phosphorylation (p-AKT at Ser473) in adipocytes. Cyclic stretching at 5% strain and 1 Hz frequency was applied to differentiated 3T3-L1 adipocytes for 2 h. ( A ) Immunoblots of AKT and p-AKT with GAPDH loading control. ( B ) After normalization with GAPDH, relative immunoblot band intensities of p-AKT/AKT were obtained. Mean ± SEM; *: p < 0.05 (n = 3) by Student t -test.
    Figure Legend Snippet: Cyclic mechanical stretch loading induces AKT phosphorylation (p-AKT at Ser473) in adipocytes. Cyclic stretching at 5% strain and 1 Hz frequency was applied to differentiated 3T3-L1 adipocytes for 2 h. ( A ) Immunoblots of AKT and p-AKT with GAPDH loading control. ( B ) After normalization with GAPDH, relative immunoblot band intensities of p-AKT/AKT were obtained. Mean ± SEM; *: p < 0.05 (n = 3) by Student t -test.

    Techniques Used: Western Blot, Control



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    Cyclic mechanical stretch loading induces <t>AKT</t> phosphorylation (p-AKT at <t>Ser473)</t> in adipocytes. Cyclic stretching at 5% strain and 1 Hz frequency was applied to differentiated 3T3-L1 adipocytes for 2 h. ( A ) Immunoblots of AKT and p-AKT with GAPDH loading control. ( B ) After normalization with GAPDH, relative immunoblot band intensities of p-AKT/AKT were obtained. Mean ± SEM; *: p < 0.05 (n = 3) by Student t -test.
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    Image Search Results


    Cyclic mechanical stretch loading induces AKT phosphorylation (p-AKT at Ser473) in adipocytes. Cyclic stretching at 5% strain and 1 Hz frequency was applied to differentiated 3T3-L1 adipocytes for 2 h. ( A ) Immunoblots of AKT and p-AKT with GAPDH loading control. ( B ) After normalization with GAPDH, relative immunoblot band intensities of p-AKT/AKT were obtained. Mean ± SEM; *: p < 0.05 (n = 3) by Student t -test.

    Journal: Bioengineering

    Article Title: Mechanical Stretch Control of Adipocyte AKT Signaling and the Role of FAK and ROCK Mechanosensors

    doi: 10.3390/bioengineering11121279

    Figure Lengend Snippet: Cyclic mechanical stretch loading induces AKT phosphorylation (p-AKT at Ser473) in adipocytes. Cyclic stretching at 5% strain and 1 Hz frequency was applied to differentiated 3T3-L1 adipocytes for 2 h. ( A ) Immunoblots of AKT and p-AKT with GAPDH loading control. ( B ) After normalization with GAPDH, relative immunoblot band intensities of p-AKT/AKT were obtained. Mean ± SEM; *: p < 0.05 (n = 3) by Student t -test.

    Article Snippet: Antibodies for AKT (MAB2055, R&D Systems, Minneapolis, TX, USA), phospho-AKT (p-AKT at Ser473) (AF887, R&D Systems, Minneapolis, TX, USA), GLUT4 (G4173, Sigma, Louis, MO, USA), and GAPDH (SC-32233, Santa Cruz, Dallas, TX, USA) were used.

    Techniques: Western Blot, Control