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p akt  (R&D Systems)


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    Structured Review

    R&D Systems p akt
    P Akt, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 108 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/af887/Human%2FMouse%2FRat+Phospho-Akt+(S473)+Pan+Specific+Antibody/pm41812059-59-196-200
    Average 94 stars, based on 108 article reviews
    p akt - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Software:

    Article Title: Placenta-specific protein 1 promotes cell proliferation and invasion in non-small cell lung cancer
    Article Snippet: Cells protein lysates (Millipore) were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred to 0.22 μm NC membranes (Sigma, St. Louis, MO, USA), and incubated with specific antibodies. .. Enhanced chemiluminescence (ECL) chromogenic substrate (Beyotime, Shanghai, China) was used to visualize the bands and the intensity of the bands were quantified by densitometry (Quantity One Software; Bio-Rad, Hercules, CA, USA). β-actin antibody (1:5,000) was used as a control, and anti-PLAC1 (1:500) (ab117528), anti-vimentin (1:2,000) (AF2105), anti-AKT (1:5,000) (MAB2055), anti-pAKT (S473) (1:2,000) (AF887) and anti-E-cadherin (1:10,000) (AF648) were purchased from R&D Systems. .. Donkey anti-goat IgG-HRP (1:5,000) (sc-2020) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

    Control:

    Article Title: Placenta-specific protein 1 promotes cell proliferation and invasion in non-small cell lung cancer
    Article Snippet: Cells protein lysates (Millipore) were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred to 0.22 μm NC membranes (Sigma, St. Louis, MO, USA), and incubated with specific antibodies. .. Enhanced chemiluminescence (ECL) chromogenic substrate (Beyotime, Shanghai, China) was used to visualize the bands and the intensity of the bands were quantified by densitometry (Quantity One Software; Bio-Rad, Hercules, CA, USA). β-actin antibody (1:5,000) was used as a control, and anti-PLAC1 (1:500) (ab117528), anti-vimentin (1:2,000) (AF2105), anti-AKT (1:5,000) (MAB2055), anti-pAKT (S473) (1:2,000) (AF887) and anti-E-cadherin (1:10,000) (AF648) were purchased from R&D Systems. .. Donkey anti-goat IgG-HRP (1:5,000) (sc-2020) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).



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